Gallery
This movie begins with a DIC image of KV, then shifts to the fluorescent channel showing red fluorescent microbeads injected into the fluid-filled lumen of KV (outlined in white). The microbeads reveal directional (counterclockwise) fluid flow in KV.
Movie 3. Cilia-Driven Directional Fluid Flow in Kupffer’s Vesicle (KV)
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Movie 2. Cilia beating in the Zebrafish Ciliated Organ Kupffer’s Vesicle
This is a differential interference contrast (DIC) image showing several beating cilia in Kupffer’s vesicle. Playback speed is significantly slower than real-time.
Movie 1. The Zebrafish Embryonic Heart
Green fluorescent proteins (GFP) expressed specifically in the heart provides an excellent tool to study embryonic heart development. This movie was made by Vanessa French, a visiting student from Erasmus Medical Center, Rotterdam, Netherlands.
In addition to Kupffer’s vesicle, we are interested in the development of other ciliated tissues. This movie shows beating ependymal cilia in the spinal cord, which moves cerebrospinal fluid.
Movie 4. Zebrafish Ependymal Cilia
Movie 5. Visualization of Cell Dynamics inside Kupffer’s Vesicle
Transgenic zebrafish that express membrane-localized green fluorescent protein (generated by Michael Tsang’s group at U. of Pittsburgh) allow us to observe changes in cell shapes in live embryos.